Human Histone H4 (acetyl K8) peptide (ab15824)

Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Modifications
      acetyl K8

Specifications

Our Abpromise guarantee covers the use of ab15824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • Histone gene cluster 1, H4A
    • Histone gene cluster 2, H4
    • dJ160A22.1
    • dJ160A22.2
    • dJ221C16.1
    • dJ221C16.9
    • FO108
    • H4
    • H4 histone family, member A
    • H4 histone family, member B
    • H4 histone family, member C
    • H4 histone family, member D
    • H4 histone family, member E
    • H4 histone family, member G
    • H4 histone family, member H
    • H4 histone family, member I
    • H4 histone family, member J
    • H4 histone family, member K
    • H4 histone family, member M
    • H4 histone family, member N
    • H4 histone, family 2
    • H4/A
    • H4/B
    • H4/C
    • H4/D
    • H4/E
    • H4/G
    • H4/H
    • H4/I
    • H4/J
    • H4/K
    • H4/M
    • H4/N
    • H4/O
    • H4/p
    • H4_HUMAN
    • H4F2
    • H4F2iii
    • H4F2iv
    • H4FA
    • H4FB
    • H4FC
    • H4FD
    • H4FE
    • H4FG
    • H4FH
    • H4FI
    • H4FJ
    • H4FK
    • HIST1 cluster, H4A
    • HIST1 cluster, H4B
    • HIST1 cluster, H4D
    • HIST2H4
    • Hist4 cluster, H4
    • Hist4h4
    • histone 1, H4a
    • histone 1, H4c
    • histone 1, H4d
    • histone 1, H4f
    • histone 1, H4h
    • histone 1, H4i
    • histone 1, H4j
    • histone 1, H4k
    • histone 1, H4l
    • histone 2, H4a
    • histone 2, H4b
    • Histone 4 family, member M
    • histone 4, H4
    • histone cluster 1, H4
    • histone cluster 1, H4a
    • histone cluster 1, H4b
    • histone cluster 1, H4c
    • histone cluster 1, H4d
    • histone cluster 1, H4e
    • histone cluster 1, H4f
    • histone cluster 1, H4h
    • histone cluster 1, H4i
    • histone cluster 1, H4j
    • histone cluster 1, H4k
    • histone cluster 1, H4l
    • histone cluster 2, H4a
    • histone cluster 2, H4b
    • histone cluster 4, H4
    • Histone family, member A
    • Histone family, member B
    • Histone family, member D
    • Histone family, member H
    • Histone family, member I
    • Histone family, member L
    • Histone gene cluster 1, H4
    • Histone gene cluster 1, H4D
    • Histone gene cluster 1, H4E
    • Histone gene cluster 1, H4K
    • Histone gene cluster 4, H4
    • Histone gene cluster 4, H4 histone
    • Histone H4
    • histone IV, family 2
    see all
  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H4 family.
  • Post-translational
    modifications
    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt

References

ab15824 has not yet been referenced specifically in any publications.

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