Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • AccessionP62805
    • SpeciesHuman

Associated products

Specifications

Our Abpromise guarantee covers the use of ab4559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • dJ160A22.1
    • dJ160A22.2
    • dJ221C16.1
    • dJ221C16.9
    • FO108
    • H4
    • H4.k
    • H4/a
    • H4/b
    • H4/c
    • H4/d
    • H4/e
    • H4/g
    • H4/h
    • H4/I
    • H4/j
    • H4/k
    • H4/m
    • H4/n
    • H4/p
    • H4_HUMAN
    • H4F2
    • H4F2iii
    • H4F2iv
    • H4FA
    • H4FB
    • H4FC
    • H4FD
    • H4FE
    • H4FG
    • H4FH
    • H4FI
    • H4FJ
    • H4FK
    • H4FM
    • H4FN
    • H4M
    • HIST1H4A
    • HIST1H4B
    • HIST1H4C
    • HIST1H4D
    • HIST1H4E
    • HIST1H4F
    • HIST1H4H
    • HIST1H4I
    • HIST1H4J
    • HIST1H4K
    • HIST1H4L
    • HIST2H4
    • HIST2H4A
    • Hist4h4
    • Histone 1 H4a
    • Histone 1 H4b
    • Histone 1 H4c
    • Histone 1 H4d
    • Histone 1 H4e
    • Histone 1 H4f
    • Histone 1 H4h
    • Histone 1 H4i
    • Histone 1 H4j
    • Histone 1 H4k
    • Histone 1 H4l
    • Histone 2 H4a
    • histone 4 H4
    • Histone H4
    • MGC24116
    see all
  • FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similaritiesBelongs to the histone H4 family.
  • Post-translational
    modifications
    Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
    Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
    Sumoylated, which is associated with transcriptional repression.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt

References for Human Histone H4 peptide (ab4559)

This product has been referenced in:
  • Sautel CF  et al. SET8-mediated methylations of histone H4 lysine 20 mark silent heterochromatic domains in apicomplexan genomes. Mol Cell Biol 27:5711-24 (2007). Read more (PubMed: 17562855) »

See 1 Publication for this product

Product Wall

ab112431is the full length protein of 103 amino acids, whereas ab4559 is only a peptide. A peptide is defined as a short polymer of less than 50 amino acids. The sequence of ab4559can be foundfrom position 50 AA onward, but that doesn't mean that the p...

Read More
Application Other

Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.

No primary antibody directed towards Histone H4 was used in this dot blot assay. This peptide was chosen to ensure there was no cross reactivity between the antibodies we are using on a regular basis. We have yet to see any cross reactivity with this peptide using 12+ antibodies directed towards histone H3 modified antibodies (some from Abcam some not).
Sample: Human Purified protein

Primary antibody (in addition to 'Histone H4 peptide - unmodified')
Primary antibody: None used

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000
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Abcam user community

Verified customer

Submitted Nov 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"