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Abcam’s Human Anti-HSP60 IgG/A/M in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Anti-HSP60 in Human Serum. This assay allows determination of IgG, IgA and IgM antibodies (total) to Human Hsp60 in serum.
A recombinant Human Hsp60 protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. An anti-Human GAM-HRP conjugated antibody is then added and incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
HSP60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. HSP60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of HSP60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.
Plates are not provided
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 100ml|
|Anti-Human GAM-HRP Conjugate||1 x 10ml|
|Human Anti-HSP60 IgG/A/M Microplate (12 x 8 wells)||1 unit|
|Human Anti-HSP60 Standard||1 x 0.12ml|
|Sample Diluent 1||1 x 100ml|
|Stop Solution 2||1 x 10ml|
|TMB Substrate||1 x 10ml|
Our Abpromise guarantee covers the use of ab133059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab133059 has not yet been referenced specifically in any publications.