Overview

  • Product name
    Human Anti-HSP60 IgG/A/M ELISA Kit
    See all Hsp60 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 16 < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall 16 < 10%
  • Sample type
    Serum
  • Assay type
    Indirect
  • Sensitivity
    2.88 ng/ml
  • Range
    7.81 ng/ml - 250 ng/ml
  • Assay time
    1h 15m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Anti-HSP60 IgG/A/M in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Anti-HSP60 in Human Serum. This assay allows determination of IgG, IgA and IgM antibodies (total) to Human Hsp60 in serum.

    A recombinant Human Hsp60 protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. An anti-Human GAM-HRP conjugated antibody is then added and incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.

  • Notes

    HSP60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. HSP60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of HSP60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.

     

    Plates are not provided

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 100ml
    Anti-Human GAM-HRP Conjugate 1 x 10ml
    Human Anti-HSP60 IgG/A/M Microplate (12 x 8 wells) 1 unit
    Human Anti-HSP60 Standard 1 x 0.12ml
    Sample Diluent 1 1 x 100ml
    Stop Solution 2 1 x 10ml
    TMB Substrate 1 x 10ml
  • Research areas
  • Function
    Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
  • Involvement in disease
    Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
  • Sequence similarities
    Belongs to the chaperonin (HSP60) family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Alternative names
    • 60 kDa chaperonin
    • 60 kDa heat shock protein, mitochondrial
    • CH60_HUMAN
    • Chaperonin 60
    • Chaperonin, 60-KD
    • CPN60
    • fa04a05
    • GROEL
    • heat shock 60kDa protein 1 (chaperonin)
    • Heat shock protein 1 (chaperonin)
    • Heat shock protein 60
    • Heat shock protein 65
    • heat shock protein family D (Hsp60) member 1
    • HLD4
    • Hsp 60
    • HSP 65
    • HSP-60
    • HSP60
    • HSP65
    • HSPD1
    • HuCHA60
    • Mitochondrial matrix protein P1
    • P60 lymphocyte protein
    • short heat shock protein 60 Hsp60s1
    • SPG13
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab133059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133059.

Protocols

References

ab133059 has not yet been referenced specifically in any publications.

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