Overview

  • Product name
    Human HVEM ELISA Kit (TNFRSF14 )
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 30 pg/ml
  • Range
    32.77 pg/ml - 8000 pg/ml
  • Recovery

    88 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 85.34 69% - 98%
    Serum 90.17 81% - 97%
    Plasma 90.83 73% - 112%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s HVEM Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HVEM in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human HVEM coated on a 96- well plate. Standards and samples are pipetted into the wells and HVEM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HVEM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HVEM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Human HVEM 2 vials
    HVEM Microplate (12 strips x 8 wells) 1 x 96 tests
    Recombinant Human HVEM Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
  • Tissue specificity
    Widely expressed, with the highest expression in lung, spleen and thymus.
  • Sequence similarities
    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • CD270
    • Herpes virus entry mediator A
    • Herpesvirus entry mediator A
    • HveA
    • TNFRSF14
    • TNR14_HUMAN
    • TR2
    • Tumor necrosis factor receptor superfamily member 14
    • Tumor necrosis factor receptor-like 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab113335

Protocols

References

ab113335 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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