Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionP55008
    • SpeciesHuman
    • SequenceMKHHHHHHASQTRDLQGGKAF­GLLKAQQEERLDE­INKQFLDDPKYSSD ED­LPSKLEGFKEKYMEF­DLNGNGDIDIMSLKRMLEK­LGVPKTHLELK KLI­GEVSSGSGETFSYP­DFLRMMLGKRSAIL­KMILMYEEKAREKEK­ PTGPPAKKAISELP
    • Molecular weight18 kDa including tags
    • Amino acids2 to 147
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab117478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 90 % by SDS-PAGE.

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    pH: 7.50
    Constituents: 0.32% Tris, 0.29% Sodium chloride

  • ReconstitutionAdd 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles.
    Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable for 24 months when stored at -20°C.

General info

  • Alternative names
    • AIF 1
    • AIF-1
    • Aif1
    • AIF1 protein
    • AIF1_HUMAN
    • Allograft inflammatory factor 1
    • Allograft inflammatory factor 1 splice variant G
    • allograft inflammatory factor-1 splice variant Hara-1
    • balloon angioplasty responsive transcription
    • BART 1
    • G1
    • G1 putative splice variant of allograft inflamatory factor 1
    • IBA 1
    • IBA1
    • interferon gamma responsive transcript
    • Interferon responsive transcript 1
    • interferon responsive transcript factor 1
    • Ionized calcium binding adapter molecule 1
    • ionized calcium-binding adapter molecule
    • Ionized calcium-binding adapter molecule 1
    • IRT 1
    • IRT1
    • Microglia response factor
    • MRF1
    • Protein g1
    see all
  • FunctionActin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • Tissue specificityDetected in T-lymphocytes and peripheral blood mononuclear cells.
  • Sequence similaritiesContains 2 EF-hand domains.
  • Post-translational
    modifications
    Phosphorylated on serine residues.
  • Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Target information above from: UniProt accession P55008 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Human Iba1 full length protein (ab117478)

ab117478 has not yet been referenced specifically in any publications.

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