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|Sample type||Average %||Range|
|Cell culture supernatant||97.37||89% - 107%|
|Serum||97.48||88% - 106%|
|Plasma||98.67||90% - 107%|
Abcam’s ICAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human soluble ICAM1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human ICAM1 ELISA Kit (CD54) (ab174445) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|340X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human ICAM1 (lyophilized)||2 vials|
|ICAM1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human ICAM1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"