• Product nameHuman ICAM1 ELISA Kit (CD54)
    See all ICAM1 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 150 pg/ml
  • Range
    150 pg/ml - 20000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.37 89% - 107%
    Serum 97.48 88% - 106%
    Plasma 98.67 90% - 107%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ICAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human soluble ICAM1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
  • Cellular localizationMembrane
  • Alternative names
    • BB2
    • CD 54
    • CD54
    • CD54 antigen
    • Cell surface glycoprotein P3.58
    • Human rhinovirus receptor
    • ICAM 1
    • Intercellular adhesion molecule 1
    • Major group rhinovirus receptor
    • MALA2
    • MGC6195
    • MyD10
    • P3.58
    • Surface antigen of activated B cells
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human ICAM1 ELISA Kit (CD54) images

  • Representative standard curve using ab100640

  • Representative standard curve using ab100640


References for Human ICAM1 ELISA Kit (CD54) (ab100640)

This product has been referenced in:
  • Mascarenhas-Melo F  et al. Diabetes abrogates sex differences and aggravates cardiometabolic risk in postmenopausal women. Cardiovasc Diabetol 12:61 (2013). Read more (PubMed: 23570342) »
  • Mascarenhas-Melo F  et al. Emergent Biomarkers of Residual Cardiovascular Risk in Patients with Low HDL-c and/or High Triglycerides and Average LDL-c Concentrations: Focus on HDL Subpopulations, Oxidized LDL, Adiponectin, and Uric Acid. ScientificWorldJournal 2013:387849 (2013). Read more (PubMed: 24319364) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

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Thank you for your enquiry and your interest in our products.

The capture antibody of the ab100640 (ICAM1 Human ELISA Kit - 1 x 96) kit is a monoclonal mouse IgG1.

If you need any further assistance in the future, please do not hesi...

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Thank you for contacting us. Here is the requested information: ab46087: Normal values for TNFa in such samples are negatives or very low, so we usually recommend to assay the human serum samples neat with Standard buffer, human serum to dilute t...

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Please accept my apologies for the miscommunication in the previous email about ab100640, the ICAM1 Human ELISA Kit. Yes, you would need to do a dilution of your samples, so that they would be within the detection range of the kit. Please let me know...

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Thank you for contacting Abcam. I have received a reply from the lab about ab100640, the ICAM1 Human ELISA Kit. Based on testing and the reported literature, normal levels of soluble ICAM in serum look to be in the low-to-mid ng/ml levels and not low p...

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