Overview

  • Product name
    Human ICAM2 ELISA Kit (CD102)
    See all ICAM2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 23.62U/ml 0.277 1.17%
    B 8 2.96U/ml 0.86 2.9%
    Inter-assay
    Sample n Mean SD CV%
    A 33 23.26U/ml 0.668 2.87%
    B 33 3.12 0.218 6.98%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 0.2 U/ml
  • Range
    0.75 U/ml - 24 U/ml
  • Assay time
    1h 40m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human ICAM2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble Inter Cellular Adhesion Molecule -2 (ICAM2) in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for ICAM2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM2 present in the samples.

    This kit will recognize both endogenous and recombinant Human ICAM2.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-ICAM2 Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    ICAM2 Microplate (12 x 8 well strips) 1 unit 2 units
    ICAM2 Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Relevance
    ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • Cellular localization
    Type 1 membrane protein
  • Alternative names
    • CD102
    • ICAM 2
    • Intercellular adhesion molecule 2
    • Ly60
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab46080

Protocols

References

ab46080 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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