Overview

  • Product name
    Human ICAM2 Matched Antibody Pair Kit
    See all ICAM2 kits
  • Detection method
    Colorimetric
  • Assay type
    ELISA set
  • Range
    187.5 pg/ml - 12000 pg/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant human ICAM2.


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab212164) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human ICAM2 Capture Antibody 1 x 100µg 1 x 20µg
    Human ICAM2 Detector Antibody 1 x 25µg 1 x 5µg
    Human ICAM2 Lyophilized Protein 1 vial 1 vial
  • Research areas
  • Relevance
    ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • Cellular localization
    Membrane; Single-pass type I membrane protein
  • Alternative names
    • CD102
    • CD102 antigen
    • ICAM 2
    • Intercellular adhesion molecule 2
    • Ly60
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab217614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab217614 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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