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|Sample type||Average %||Range|
|Serum||92||89% - 98%|
|Cell culture media||95||92% - 99%|
|Heparin Plasma||97||87% - 115%|
|EDTA Plasma||101||89% - 122%|
|Citrate Plasma||84||77% - 88%|
Quantify up to 75 proteins and peptides per well, using just 12.5 µL of sample, with FirePlex® immunoassays. The assay takes approximately 3.5 hours, followed by particle analysis using a compatible flow cytometer and data analysis using our free FirePlex Analysis Workbench software.
Benefits of the assay
- Quantify both low and high abundance analytes with a typical sensitivity of 0.5 pg /mL and a fully extended dynamic range spanning 4.5 logs
- Choose from hundreds of analytes to easily build your own custom panel, use a convenient pre-mixed pre-designed panel, or add new analytes to a pre-designed panel
- Consistent batch-to-batch performance with assays built using the same recombinant monoclonal antibody pairs as our SimpleStep ELISA® and Matched Antibody Pair Kits
- One FirePlex particle platform for both multiplex miRNA assays and multiplex immunoassays
Find out more about multiplex immunoassays using FirePlex particles here
Important - Use of the FirePlex Multiplex Immunoassay Kit also requires the purchase of:
- a) Human Multiplex Immunoassay Core Kit, ab208203; and
- b) Multiplex Immunoassay Protein Standard Mix, such as ab218999.
FirePlex® is a registered trademark in the United States and is an unregistered trademark elsewhere.
The following information provides the sensitivity range and recommended starting dilutions for validated biological samples.
Sensitivity range - 21.5 - 23.8 pg/mL
|Recommended Dilution Range|
|Components||Identifier||1 x 96 tests|
|75X Human ICAM2 Capture Particles||Particle Code - 18||1 unit|
|Human ICAM2 Biotin Detector (Lyophilized)||1 unit|
Mean background-subtracted data values are graphed (+/- SD).
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM2 concentration was determined to be 61.8 ng/mL with a range of 23.2-91.8 ng/mL.
The concentrations of ICAM2 were interpolated from the ICAM2 standard curves and corrected for sample dilution. 1X diluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (heparin) 25%, and plasma (citrate) 25%. The interpolated dilution factor corrected mean values are plotted. The mean ICAM2 concentration was determined to be 120 ng/mL in serum, 80.2 ng/mL in plasma (EDTA), 39.2 ng/mL in plasma (heparin), and 53.8 ng/mL in plasma (citrate).
The concentrations of ICAM2 were interpolated from the ICAM2 standard curves and corrected for sample dilution. 1X diluted samples are as follows: 25% urine, 25% milk (defatted), 25% cerebrospinal fluid (CSF), 25% synovial fluid (SF), and 25% bronchial lavage (BAL). The interpolated dilution factor corrected values are plotted. The mean ICAM2 concentration was determined to be 824 pg/mL in urine, 1348 pg/mL in milk (defatted), 483 pg/mL in cerebrospinal fluid (CSF), 31378 pg/mL in synovial fluid (SF), and 1370 pg/mL in bronchial lavage (BAL). Saliva was tested and measured less than the lowest human ICAM2 standard, 123.46 pg/mL.
The concentrations of ICAM2 were interpolated from the ICAM2 standard curves and corrected for sample dilution. 1X diluted samples are as follows: 25% 10F HGDMEM media. The interpolated dilution factor corrected mean values are plotted. The mean ICAM2 concentration was determined to be 66.1 ng/mL in 10F HGDMEM media.
ab219017 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"