• Product nameHuman ICAM3 ELISA Kit (CD50)
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Sample A 8 19.75ng/ml 0.172 0.86%
    Sample B 8 2.83ng/ml 0.099 3.49%
    Sample n Mean SD CV%
    Sample A 36 19.51ng/ml 0.391 1.99%
    Sample B 36 2.71ng/ml 0.176 6.27%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.3 ng/ml
  • Range
    0.62 ng/ml - 20 ng/ml
  • Assay time
    1h 40m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ICAM3 (CD50) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM3 in serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM3 present in the samples. 

    This kit will recognize both endogenous and recombinant Human ICAM3.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-ICAM-3 (CD50) Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    ICAM3 Control Silver 2 vials 4 vials
    ICAM3 Microplate (12 x 8 well strips) 1 unit 2 units
    ICAM3 Standard Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • RelevanceICAM3 is a member of the intercellular adhesion molecule family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
  • Alternative names
    • CD50
    • CDW50
    • ICAM 3
    • ICAM R
    • ICAMR
    • Intercellular adhesion molecule 3
    see all
  • Database links


Our Abpromise guarantee covers the use of ab46024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human ICAM3 ELISA Kit (CD50) images

  • Representative Standard Curve using ab46024


References for Human ICAM3 ELISA Kit (CD50) (ab46024)

ab46024 has not yet been referenced specifically in any publications.

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The expiration date that we assigned for that lot is October 2013. However, our guarantee is in effect for only 6 months after the purchase of the kit, so I recommend contacting us immediately if you have any iss...

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The lab let me know that it is not necessary to use glass vials. Plastic vials suit perfectly. The crucial point is cleanness.

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