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Abcam’s IFN gamma Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in supernatants, buffered solutions or serum and plasma samples and other body fluids.
A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.
This kit will recognize both endogenous and recombinant Human IFN gamma.
Get higher sensitivity in only 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7ml||1 x 13ml|
|Biotinylated anti-IFN gamma||Red||1 x 400µl||2 x 400µl|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|Control||Silver||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|IFN gamma Microplate (12 x 8 well strips)||1 unit||2 units|
|IFN gamma Standard (Lyophilized)||Yellow||2 vials||4 vials|
|Standard Diluent (Serum)||1 x 7ml||2 x 7ml|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative Standard Curve using ab46025
ab46025 has not yet been referenced specifically in any publications.