Human IFN gamma High Sensitivity ELISA Kit (ab46048)

Overview

  • Product nameHuman IFN gamma High Sensitivity ELISA Kit
    See all Interferon gamma kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.69 pg/ml
  • Range
    0.78 pg/ml - 25 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108 % - %
    Serum 92 % - %
    Plasma 72 % - %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IFN gamma Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in Human sera, plasmas, buffered solutions and cell culture media.

    A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples. 

    This kit will recognize both endogenous and recombinant Human IFN gamma.

     

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceMammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFN Immune
    • IFNG
    • Interferon gamma
    • Interferon Gamma Precursor
    • Macrophage Activating Factor
    • MAF
    • T Cell Interferon
    • Type II Interferon
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IFN gamma High Sensitivity ELISA Kit images

  • IFN gamma measured in cell culture supernatants from control or treated (48 hours; 2% PHA-M; LifeTechnologies) PBMCs, diluted 1/100-1/10000 (duplicates +/- SD).

  • IFN gamma measured in undiluted cell culture supernatants from control or stimulated (48 hours with 50 ng x mL-1 PMA (ab120297) and 1 μM ionomycin (ab120116)) Jurkat cells. Control sample was below limit of detection (<0.57 pg x mL-1)  (duplicates +/- SD).

  • IFN gamma measured in undiluted human biological fluids; mouse and rat samples (plasma and serum) were below limit of detection (duplicates +/- SD).

  • Dilution curves of IFN gamma (ab46048) and NIBSC standard (87/586). One ng of standard IFN gamma corresponds to 12.3 (+/- 1.3) IU NIBSC 87/586. Background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab46048

Protocols

References for Human IFN gamma High Sensitivity ELISA Kit (ab46048)

ab46048 has not yet been referenced specifically in any publications.

Product Wall

ab46048, human High Sensitivity IFNg ELISA kit, does not cross react with mouse sample.

Thank you very much for contacting us with your question.

We don't assign specific expiration dates for our products, but we expect the kit ab46048 to be viable for 12-18 months. I've looked at the specific lots on our most recent orders to y...

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Thank you for contacting us with your questions, and for your patience while I have been in touch with the lab regarding your enquiries.
The lab scientists do not recommend storing the reconstituted standard because it is not stable and it may not ...

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Thank you for contacting us.

I have confirmed that the standard for the Interferon Gamma Human ELISA Kit, ab46048, is not available as a stand-alone item.

The laboratory that developed the assay warns against storing the standard:...

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Thank you for your patience. I have checked on the bovine IFN-gamma ELISA Kit. Unfortunately, the kit currently is not available in our catalog as the quality control tests have not yet been completed.  In general, due to the low homology between...

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Thank you for contacting us. I can confirm that ab46087 can be used to detect TNF alpha in human plasma samples. The cost per KIT can be obtained from our Australian distributor Sapphire Bioscience. Their address is as follows. Please discuss with ...

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Thanks for your enquiry. I am very sorry to hear that you are having problems with this product. I have been discussing this enquiry with my colleagues in the Lab and would like to share the followings. 1) First, the regular volume of HRP diluent...

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