Anti-Human Ig light chain antibody [7A9] (ab1942)

Overview

  • Product nameAnti-Human Ig light chain antibody [7A9]
    See all Human Ig light chain primary antibodies
  • Description
    Mouse monoclonal [7A9] to Human Ig light chain
  • SpecificityHuman IgA, IgG and IgM.
  • Tested applicationsSuitable for: Indirect ELISA, Conjugation, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Mixture of light chains of human immunoglobulin.

  • Positive control
    • IHC-P: FFPE human spleen tissue sections.
  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurity tested by electrophoresis.
  • ClonalityMonoclonal
  • Clone number7A9
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1e+006.
Conjugation Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceThere are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
  • Cellular localizationCell Membrane: single-pass type I membrane protein and Secreted
  • Database links
  • Alternative names
    • HCAK1 antibody
    • Ig kappa chain antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • immunoglobulin kappa light chain (VJ) antibody
    • kappa 1 immunoglobulin light chain antibody
    • KM antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Anti-Human Ig light chain antibody [7A9] images

  • IHC image of Human Ig light chain staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1942, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Human Ig light chain antibody [7A9] (ab1942)

ab1942 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 7 µg
Gel Running Conditions Non-reduced Denaturing (4-12% Bis Tris NuPage)
Sample Human Recombinant protein (Monoclonal human IgG antibody produced in lab)
Specification Monoclonal human IgG antibody produced in lab
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Nov 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"