Recombinant

Anti-Human Ig light chain antibody [RM129] (Biotin) (ab193185)

Overview

  • Product name
    Anti-Human Ig light chain antibody [RM129] (Biotin)
    See all Human Ig light chain primary antibodies
  • Description
    Rabbit monoclonal [RM129] to Human Ig light chain (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cyt, Sandwich ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Goat
  • Immunogen

    Full length protein corresponding to Human IgG. Purified Native Human IgG from Human Plasma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 50% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Protein A affinity purified from an animal origin–free culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    RM129
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 0.02 - 0.25 µg/ml.

May be paired with ab195574.

ICC/IF Use a concentration of 0.5 - 2 µg/ml.
IHC-P Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance
    There are only two types of light chain: kappa and lambda in mammals. Other types of light chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei. In each antibody, only one type is present and the two chains are identical. Each light chain has two successive domains: one constant and one variable domain. In humans 60% of light chains are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC concentrations can provide a rapid and sensitive indication of response to treatment.
  • Cellular localization
    Cell Membrane: single-pass type I membrane protein and Secreted
  • Database links
  • Alternative names
    • HCAK1 antibody
    • Ig kappa chain antibody
    • IGKC antibody
    • Immunoglobulin kappa constant antibody
    • immunoglobulin kappa light chain (VJ) antibody
    • kappa 1 immunoglobulin light chain antibody
    • KM antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • A titer Sandwich ELISA using ab193185 as the detection antibody. The plate, coated with the capture antibody anti-human IgG, was loaded with different amounts of human IgG. A serial dilution of ab193185 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.

  • ELISA of human immunoglobulins shows ab193185 reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain. The plate was coated with 50ng/well of different immunglobulins. 100 ng/mL or 20 ng/mL was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

References

ab193185 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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