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|Sample type||Average %||Range|
|Saliva||90.31||87.91% - 92.45%|
|Milk||92.21||81% - 101.51%|
|Urine||99.48||86.21% - 109.34%|
|Serum||98.37||81.35% - 109.17%|
|Cell culture media||100.65||85.17% - 117.29%|
|Heparin Plasma||97.17||91.05% - 109.18%|
|EDTA Plasma||81.23||75.01% - 87.76%|
|Citrate Plasma||93.47||88.78% - 98.99%|
Abcam’s Human IgA in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgA protein in Human serum, plasma, milk, urine, saliva, and culture media samples.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Human Immunoglobulin A (IgA) belongs to the immunoglobulin family of proteins which include Human IgG, IgE, and IgM, and are known as antibodies. IgA has an essential role in mucosal immunity. IgA is the second most common serum Ig and is about one-fifth of the concentration of IgG, however it is the most abundant immunoglobulin in secretions, such as saliva, mucus, sweat, and tears. In normal human serum, IgA is found mainly as a monomeric, alternatively, in secretions, IgA is found as a dimer containing two additional proteins, secretory piece, and a J chain. The secretory piece is synthesized in the epithelial cells and is added to IgA as it passes into the secretions, protecting it from degradation. IgA works is with lysozymes to hydrolyze carbohydrates in bacterial cells walls to clear the infection.
The most common disease associated with high levels of IgA in the kidney is IgA nephropathy, aka Berger’s disease. There is no clear clarification as to why IgA deposits occur in the kidney, however abnormality in the immune system is a possibility. Other diseases that IgA is associated with are Celiac disease and Henoch-Schonlein purpura (HSP).
|Components||1 x 96 tests|
|10X Human IgA Capture Antibody||1 x 600µl|
|10X Human IgA Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent CPI - HAMA Blocker (ab193969)||1 x 6ml|
|Human IgA Lyophilized Purified Protein||2 vials|
|Plate Seals||1 unit|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Sample Diluent NS||1 x 50ml|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab196263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Ten individual healthy donors were evaluated for the presence of IgA in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1:2X105). The mean level of Human IgA was found at 3.978 mg/mL with a range of 1.016 – 9.476 mg/mL.
Fluids from a pooled selection of healthy donors were evaluated for the presence of Human IgA in serum, milk, saliva, and urine using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1:2X105). The mean levels of Human IgA in serum were found at 2.45 mg/mL, in milk at 0.74 mg/mL, in saliva at 0.94 mg/mL and in urine at 0.05 mg/mL.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"