Human IgA full length protein (ab91025)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab91025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % by SDS-PAGE.

  • FormLiquid
  • Additional NotesProtein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.32 Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.05% Sodium Azide
    Constituents: 100mM Sodium chloride, 100mM Tris HCl, pH 8.0

General info

  • Alternative names
    • Hepatocellular carcinoma-associated protein TB6
    • Ig alpha 1 chain C region
    • Ig alpha 2 chain C region
    • IGHA
    • IGHA1
    • IGHA2
    • Immunoglobulin heavy constant alpha 1
    • Immunoglobulin heavy constant alpha 2
    • Immunoglobulin heavy constant alpha 2 A2m marker
    • PIgR
    • Poly-Ig receptor
    • polymeric immunoglobulin receptor
    • polymeric immunoglobulin receptor Secretory component
    see all
  • RelevanceHuman IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. OR: The secretory component is a component of immunoglobulin A (IgA) which consists of a portion of the polymeric immunoglobulin receptor. Polymeric IgA binds to the polymeric immunoglobulin receptor on the basolateral surface of epithelial cells and is taken up into the cell via transcytosis. The receptor-IgA complex passes through the cellular compartments before being secreted on the luminal surface of the epithelial cells, still attached to the receptor. Proteolysis of the receptor occurs and the dimeric IgA molecule, along with the secretory component, are free to diffuse throughout the lumen.
  • Cellular localizationSecreted

Human IgA full length protein images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 µg IgA (reduced/heated)
    Lane 2: 10 µg IgA (reduced/heated)
    Lane 3: 20 µg IgA (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 µg IgA (non-reduced/no heat)
    Lane 6: 10 µg IgA (non-reduced/no heat)
    Lane 7: 20 µg IgA (non-reduced/no heat)

References for Human IgA full length protein (ab91025)

ab91025 has not yet been referenced specifically in any publications.

Product Wall


The purification of IgA involves an ammonium sulfate precipitation as well as a series of chromatographic steps to separate IgA from the other immunoglobulins.

Based on IEP results, there are no detectable IgG, IgM, IgE, or IgD contamina...

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Yes, both proteins are essentially the same with different purity.

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Expiry date: 2012-06-04 <...

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Thank you very much for your interest in ab91025.

To our knowledge, ab91025 has not been testedused to perform a dot blot. Therefore, I can offer a discount off a future purchase if you buy ab91025 now, test it indot blotand submit feedback ...

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Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage ins...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"