Overview

  • Product name
    Human IgD ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type
    Serum, Plasma, Other biological fluids
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    4.455 ng/ml
  • Range
    6.25 ng/ml - 400 ng/ml
  • Recovery

    > 85 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IgD Human ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgD in Human samples.

    In this assay the IgD present in samples reacts with the anti-IgD antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgD antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgD. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgD in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgD in the test sample. The quantity of IgD in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab157708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab157708 IgD Human ELISA Kit.

Protocols

References

ab157708 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The IgD ELISA ab157708 comes with 12 x 8 strips in a frame, not a single solid plate.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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