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ab108650 Immunoglobulin E (IgE) Human ELISA Kit is intended for the quantitative determination of Immunoglobulin E (IgE) concentration in Human serum.
Patients with atopic allergic diseases such as atopic asthma, atopic dermatitis, and hay fever have been shown to exhibit increased total immunoglobulin E (IgE) levels in blood. IgE is also known as the reagenic antibody. In general, elevated levels of IgE indicate an increased probability of an IgE-mediated hypersensitivity, responsible for allergic reactions. Parasitic infestations such as hookworm, and certain clinical disorders including aspergillosis, have also been demonstrated to cause high levels of IgE. Decreased levels of IgE are found in cases of hypogammaglobulinemia, autoimmune diseases, ulcerative colitis, hepatitis, cancer, and malaria. Cord blood or serum IgE levels may have prognostic value in assessing the risk of future allergic conditions in children. The IgE serum concentration in a patient is dependent on both the extent of the allergic reaction and the number of different allergens to which he is sensitized. Nonallergic normal individuals have IgE concentrations that vary widely and increase steadily during childhood, reaching their highest levels at age 15 to 20, and thereafter remaining constant until about age 60 when they slowly decline.
|Components||1 x 96 tests|
|Enzyme Conjugate Reagent||1 x 18ml|
|IgE Reference Standard 1||1 x 0.5ml|
|IgE Reference Standard 2||1 x 0.5ml|
|IgE Reference Standard 3||1 x 0.5ml|
|IgE Reference Standard 4||1 x 0.5ml|
|IgE Reference Standard 5||1 x 0.5ml|
|IgE Reference Standard 6||1 x 0.5ml|
|Monoclonal anti-IgE-coated microtiter plate||1 unit|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
|Zero Buffer||1 x 13ml|
Our Abpromise guarantee covers the use of ab108650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
IgE measured in biological fluids showing quantity (IU) per mL of tested sample. Samples were diluted 1-2 fold.