Overview

  • Product name
    Human IgE ELISA Kit
    See all IgE kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.04%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.53%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.02 ng/ml
  • Range
    0.11 ng/ml - 1.33 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 108 98% - 118%
    Cell culture media 107 96% - 118%
    Heparin Plasma 100 98% - 102%
    EDTA Plasma 97 84% - 115%
    Citrate Plasma 111 102% - 116%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    IgE in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgE protein in Human serum, plasma and cell culture supernantants samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Human Immunoglobulin E (IgE) is a class of antibodies that plays a fundamental role in various allergic reactions. IgE is composed of two heavy chains and two light chains, found to be typical of Ig molecules. The epsilon heavy chain attaches to its high-affinity receptor Fc epsilon receptor (Fc?RI) found primarily on mast cells, basophils, and eosinophils. Binding of IgE to its receptor initiates a release of vasoactive amines (i.e. histamines) and other mediators of allergic responses into the surrounding tissue.

    Human IgE is produced in the lungs, skin and mucous membrane, but is typically the least abundant antibody isotope. Its main function is immunity against parasites such as parasitic worms like Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE also plays a role in type I hypersensitivity, which manifests various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergy, and some types of chronic urticaria, and atopic dermatitis.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IgE Capture Antibody 1 x 600µl
    10X Human IgE Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    ab221825 - Antibody Diluent 5BI 1 x 6ml
    Human IgE Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas
  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Secreted
  • Alternative names
    • Ig epsilon chain C region
    • IgE
    • IGHE
    • immunoglobulin heavy constant epsilon
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab195216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Three individual AD donors and NHS pooled from 50 healthy donors were evaluated for the presence of Human IgE in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1:5,000). The mean level of Human IgE was found at 950 ng/mL for AD donors with a range of 290 – 2,126.5 ng/mL. The level of Human IgE in NHS was found at 1,000 ng/mL.

Protocols

References

This product has been referenced in:
  • Sullivan KD  et al. Trisomy 21 causes changes in the circulating proteome indicative of chronic autoinflammation. Sci Rep 7:14818 (2017). Read more (PubMed: 29093484) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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