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|Sample type||Average %||Range|
|Serum||108||98% - 118%|
|Cell culture media||107||96% - 118%|
|Heparin Plasma||100||98% - 102%|
|EDTA Plasma||97||84% - 115%|
|Citrate Plasma||111||102% - 116%|
IgE in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgE protein in Human serum, plasma and cell culture supernantants samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Human Immunoglobulin E (IgE) is a class of antibodies that plays a fundamental role in various allergic reactions. IgE is composed of two heavy chains and two light chains, found to be typical of Ig molecules. The epsilon heavy chain attaches to its high-affinity receptor Fc epsilon receptor (Fc?RI) found primarily on mast cells, basophils, and eosinophils. Binding of IgE to its receptor initiates a release of vasoactive amines (i.e. histamines) and other mediators of allergic responses into the surrounding tissue.
Human IgE is produced in the lungs, skin and mucous membrane, but is typically the least abundant antibody isotope. Its main function is immunity against parasites such as parasitic worms like Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE also plays a role in type I hypersensitivity, which manifests various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergy, and some types of chronic urticaria, and atopic dermatitis.
|Components||1 x 96 tests|
|10X Human IgE Capture Antibody||1 x 600µl|
|10X Human IgE Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|ab221825 - Antibody Diluent 5BI||1 x 6ml|
|Human IgE Lyophilized Purified Protein||2 vials|
|Plate Seals||1 unit|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|Stop Solution||1 x 12ml|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab195216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Three individual AD donors and NHS pooled from 50 healthy donors were evaluated for the presence of Human IgE in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1:5,000). The mean level of Human IgE was found at 950 ng/mL for AD donors with a range of 290 – 2,126.5 ng/mL. The level of Human IgE in NHS was found at 1,000 ng/mL.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"