Human IgE full length protein (Azide-free) (ab65866)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Additional sequence informationObserved band of ~185kDa on SDS-PAGE

Specifications

Our Abpromise guarantee covers the use of ab65866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Immunocytochemistry

  • Endotoxin level< 25.000 Eu/mg
  • Purity> 90 % SDS-PAGE.
    Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
  • FormLiquid
  • Additional notes

    This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

    This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

    • Less batch-to-batch variation
    • Higher degree of purity (>90%)
    • A more uniform product (monoclonal and not polyclonal)

     

    IgE has been used in a variety of cell stimulation assays, including the following cell types:

    • Human airway smooth muscle (HASM) cells
    • mast cells (MC)
    • Human bronchial/tracheal smooth muscle (B/TSM) cells
    • acute myelogenous leukemia (AML) blasts
    • Human neutrophils

     

    This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.

     

    1 mg of ab65866 is 380,000± 50,000 IU/ml.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.15M Sodium chloride, 0.01M PBS, pH 7.4

General Info

  • Alternative names
    • Igh2
    • IGHE
    • IGHEP1
    • Immunoglobulin heavy constant epsilon
  • RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localizationCell Membrane

Human IgE full length protein (Azide-free) images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Lane 1: MW markers
    Lane 2: ab55866
    Lane 3: Native IgE purified from myeloma plasma, kappa

    IgE proteins were diluted from stock to 5µg/well. An aliquot of each protein was loaded onto 4-12% gradient and gels were subsequently run for 65 mins at 120V.

References for Human IgE full length protein (Azide-free) (ab65866)

This product has been referenced in:
  • van Beek N  et al. Correlation of Serum Levels of IgE Autoantibodies Against BP180 With Bullous Pemphigoid Disease Activity. JAMA Dermatol N/A:N/A (2016). Read more (PubMed: 27829102) »
  • Baltabekova AZh  et al. SplitCore Technology Allows Efficient Production of Virus-Like Particles Presenting a Receptor-Contacting Epitope of Human IgE. Mol Biotechnol 57:746-55 (2015). Read more (PubMed: 25837568) »

See all 3 Publications for this product

Product Wall

Application Competitive ELISA
We developed a quantitative and sensitive immunoassay to determine Human IgE in human serum using gold nanoclusters (AuNCs) as fluorescent labels. The synthesized AuNCs have a particle size of 2.7 ± 0.1 nm, a maximum fluorescence emission at 710 nm and high stability. The covalent bioconjugation of these AuNCs with the antibody was carried out by the carbodiimide reaction. A molar ratio 1:3 (antibody:AuNCs) was selected.


Human IgE from Abcam (ab65866) was used as standard to prepare the calibration curve in PBS and in human serum matrix (attached figure). Two immunoassay configurations were assayed (competitive and sandwich format), obtaining better detection limit with the competitive format (0.2 ng/mL). For both formats the detection limits were better than those obtained with current commercial ELISA kits (12 ng/mL).

Moreover, the developed fluorescent competitive immunoassay offers other interesting advantages such as fewer reagents and simpler procedures.

The applicability of this new AuNCs immunoprobe was successfully demonstrated for the determination of IgE in human serum, using the competitive configuration.

This research has been published in Biosensors and Bioelectronics,

http://dx.doi.org/10.1016/j.bios.2015.08.011

Authors: María Cruz Alonso, Laura Trapiella-Alfonso, José M Costa Fernández, Rosario Pereiro, Alfredo Sanz-Medel

Title: “Functionalized gold nanoclusters as fluorescent labels for immunoassays: Application to Human serum immunoglobulin E determination”
Username

Miss. María Cruz

Verified customer

Submitted Sep 29 2015

Abreviews
Application ELISA
The hIgE works in my assay. But the labelled concentration is different from the concentration measured by BCA method. I recommend that measuring the concenration before using it.
Username

Abcam user community

Verified customer

Submitted Sep 03 2014

The masses in the molecular weight marker are as follows, heaviest to lightest in kDa:

1. 195
2. 142
3. 96
4. 71 (Dark band)
5. 48
6. 33
7. 28
8. 22

I am sorry to confirm we are not able to sell this antibody at 1 mg/ml. I can suggest to consider trying concentrating the protein once you receive it.

Please note that as far as we are aware it has not been tested by the application you wis...

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The lab let me know that they do not measure endotoxin levels in any of their protein solutions and hence they cannot give us a specific value for ab65866.

I can confirm that ab65866 Human IgE protein has a purity of >95% as determined by SDS PAGE.
I am sorry it has not been given a purity 'grading'.

Yes, ab65866 is produced from the HE1 clone.

The antigen is produced in a monoclonal hybridoma, and is not purified from blood or anything else, so the concentration is determined by measuring OD 280. It is then sold based on mg/mL and not in units as the WHO standard product.

The ab65866 Human IgE protein is an affinity-purified, fully human IgE antibody with kappa light chains. The IgE is produced in vitro from a monoclonal hybridoma. The hybridoma is made by purifying IgE producing B cells from a patient with multiple Mye...

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Yes, this protein can stimulate mast cells.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"