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|Sample type||Average %||Range|
|Cell culture supernatant||102.5||98% - 110%|
|Serum||119.6||105% - 138%|
|Plasma||105.4||81% - 121%|
Abcam’s IGF1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IGF1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
|Components||1 x 96 tests|
|120X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|Assay Diluent C||2 x 30ml|
|Biotinylated anti-Human IGF-1||2 vials|
|IGF-1 Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Human IGF1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100545 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
Representative standard curve using ab100545.
IGF1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-64 fold.
ab100545 has not yet been referenced specifically in any publications.