Overview

  • Product name
    Human IGF1 ELISA Kit
    See all IGF1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.2 ng/ml
  • Range
    0.1 ng/ml - 30 ng/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.5 98% - 110%
    Serum 119.6 105% - 138%
    Plasma 105.4 81% - 121%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IGF1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IGF1 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


     Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100545.

  • IGF1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 1-64 fold.

  • Human IGF1 standard curve: mean of duplicates (+/- SD)

Protocols

References

This product has been referenced in:
  • Baskerville CL  et al. Comparison and validation of ELISA assays for plasma insulin-like growth factor-1 in the horse. Open Vet J 7:75-80 (2017). Read more (PubMed: 28540255) »
  • Groër MW  et al. Allostatic Perspectives in Women Veterans With a History of Childhood Sexual Assault. Biol Res Nurs 18:454-64 (2016). Read more (PubMed: 27067613) »

See all 2 Publications for this product

Customer reviews and Q&As

ab100545-Human IGF1 ELISA Kit for Human Saliva

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This kit worked successfully to quantify IGF1 protein in human saliva. We obtained valid results for all of our wells and were able to use these results to compare to them to amounts of BDNF and cortisol in saliva. The results from this kit greatly contributed to my senior thesis project, which looked at differences in IGF1 levels across a 3-month exercise intervention in older adults. We will very likely be ordering this kit again in for future analyses of IGF1.
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Abcam user community

Verified customer

Submitted Apr 14 2017

This kit is designed to measure free IGF1. IGF-1 is usually bound to IGFBPs in the serum/plasma, so if you would like to measure total IGF1, it is advisable to release the analytes by performing an extraction procedure such as acid-ethanol treatment (P...

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Unfortunately the immunogen sequence for the antibodies used in the kit ab100545 is not available. Nor do we have cross reactivity data for kangaroo species. If it helps, the capture antibody used is monoclonal and the detection antibody is polyclonal....

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L’anticorps de capture de ce kit ne reconnait pas l’IGF-1 du rat. Pour l’anticorps de détection, la réactivé avec la protéine du rat n’a pas été testé donc malheureusement, ce kit...

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The host species for the capture antibody is mouse and the detection antibody is goat.

Vielen Dank für Ihre Antwort und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.

Es tut mir leid, dass Sie Probleme mit diesem Kit hatten. Da die Standardkurve gutgelungen ist, wird höchswahr...

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Vielen Dank für Ihren Anruf.

Leider ist es so, wie ich befürchtet habe, wir können leider keine Angabe zu diesen Kits mit HepG2 Zellen, bzw mit anderen Leberzelllinien machen. Grundsätzlich spricht aber nichts gegen die Ver...

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Thank you for your enquiry.

ab100664 VEGFC Human ELISA Kit :

I can confirm this kit recognizes the mature form of VEGF-C (aa 103-227). Based on sequence similarity, the kit is also expected to detect the pro-form but this has not ...

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Thank you for contacting us. I have been in contact with the lab regarding your inquiry.They are not familiar with the Immulite 1000. However, as long as the system can perform the protocol as written, we do not see why it would not work. As this sy...

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Thank you for your inquiry.

I have contacted the lab with your enquiry and here is their advise fot you:

"What type of sample is the customer testing? If they are testing serum or plasma, are they performing the recommended ext...

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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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