Overview

  • Product nameHuman IGFBP1 ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    > 92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.64 83% - 102%
    Serum 95.27 84% - 104%
    Plasma 92.47 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IGFBP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceInsulin like growth factor binding protein 1 (IGFBP1) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The N terminal and C terminal regions are highly homologous among rat, human and bovine sequences. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner.
  • Alternative names
    • AFBP
    • hIGFBP-1
    • IBP1
    • IGF-binding protein 1
    • IGF-BP25
    • Insulin-like growth factor-binding protein 1
    • Placental protein 12
    • PP12
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP1 ELISA Kit images

  • IGFBP1 measured in cell lysates showing quantity (pg) per mg of protein

  • IGFBP1 measured in biological fluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100539

  • Representative standard curve using ab100539

Protocols

References for Human IGFBP1 ELISA Kit (ab100539)

This product has been referenced in:
  • Davila J  et al. Rac1 Regulates Endometrial Secretory Function to Control Placental Development. PLoS Genet 11:e1005458 (2015). Read more (PubMed: 26305333) »
  • DeCensi A  et al. Differential effects of metformin on breast cancer proliferation according to markers of insulin resistance and tumor subtype in a randomized presurgical trial. Breast Cancer Res Treat 148:81-90 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25253174) »

See all 3 Publications for this product

Product Wall

The immunogen used to raise the antibody pair in IGFBP1 Human ELISA Kit was a recombinant human IGFBP-1, aa 26-259. (This is the full length protein minus the signal peptide).

Unfortunately, epitope mapping has not been performed so it is not ...

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Abreviews
I tried this product on the conditioned media from the decidualized stromal cells. The product met my expectation and the protocol provided was clear and easy to use.
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Verified customer

Submitted Sep 03 2013

Vielen Dank für Ihren Anruf.

Leider ist es so, wie ich befürchtet habe, wir können leider keine Angabe zu diesen Kits mit HepG2 Zellen, bzw mit anderen Leberzelllinien machen. Grundsätzlich spricht aber nichts gegen die Ver...

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Vielen Dank für diese Rückmeldung (wir freuen uns sehr über das Lob!).

Wie besprochen, habe ich eine kostenlose Ersatzlieferung an die ursprüngliche Lieferadresse für Sie in Auftrag gegeben. Sie hat die Referenznummer...

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Es tut mir leid, dass Sie diese Probleme m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"