Overview

  • Product nameHuman IGFBP2 ELISA Kit
    See all IGFBP2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.25 86% - 105%
    Serum 95.77 84% - 103%
    Plasma 97.59 85% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IGFBP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • DomainThe C-terminus is required for IGF-binding and growth inhibition.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGF-BP53
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin Like Growth Factor Binding Protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP2 ELISA Kit images

  • Representative standard curve using ab100540

  • Representative standard curve using ab100540

Protocols

References for Human IGFBP2 ELISA Kit (ab100540)

This product has been referenced in:
  • Chang YC  et al. The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress. Front Aging Neurosci 6:191 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25136316) »
  • Sunderic M  et al. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol 58C:154-158 (2014). Read more (PubMed: 25106097) »

See all 3 Publications for this product

Product Wall

Thank you and your customer for your inquiry.

Although we have not tested the Human ab100540 and ab100544ELISA kits with ascite fluid,these kits are very accommodating to many different samples types so the ascites will most likely work.
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Thank you for contacting Abcam.

For both of these ELISA kits we do recommend that you make the wash buffer up fresh before every use and we haven't tested the long term stability of the buffer when diluted to 1X. It should be good if stored o...

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Because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products. Therefore, I am afraid I cannot send you a free sample of the IGFBP2 Human ELISA Kit ab100540.
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I apologize for the delay in getting back to you in regards to this issue, and I appreciate your patience as I have been discussing this situation with my colleagues. I can understand your frustration and disappointment as the results have been unexpec...

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Thank you very much for the data. I am working with some of my colleagues to resolve this as quickly as possible, and I appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Thank you for your reply. I have found the order with the additional kits that you mentioned. In order to process this refund, I will need a little bit more technical information. Have you tried all of these kits, or did you test 1 from each set and th...

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Thank you for your reply, and I apologize that these kits did not work well for you. I did not find the attached summary of the test results, could you please send these again? We do fully guarantee these kits to work, so I have issued credit note *** ...

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Thank you for your patient. I just had a response from my lab colleagues. They confirmed that all three kits were designed against the mature form of the protein (which is the full amino acid sequence minus the signalling peptide). As the epito...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"