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|Sample type||Average %||Range|
|Cell culture supernatant||71.5||65% - 92%|
|Serum||94.3||81% - 103%|
|Plasma||91.6||70% - 100%|
Abcam’s IGFBP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP5 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IGFBP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent D||1 x 15ml|
|700X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Human IGFBP5||2 vials|
|IGFBP5 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human IGFBP5 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100543 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100543 has not yet been referenced specifically in any publications.