• Product nameHuman IGFBP5 ELISA Kit
    See all IGFBP5 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 ng/ml
  • Range
    1.1 ng/ml - 800 ng/ml
  • Recovery

    89 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 71.5 65% - 92%
    Serum 94.3 81% - 103%
    Plasma 91.6 70% - 100%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IGFBP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Tissue specificityOsteosarcoma, and at lower levels in liver, kidney and brain.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • IBP 5
    • IBP-5
    • IBP5
    • IBP5_HUMAN
    • IGF binding protein 5
    • IGF BP5
    • IGF-binding protein 5
    • IGFBP 5
    • IGFBP-5
    • IGFBP5
    • Insulin like growth factor binding protein 5
    • Insulin-like growth factor-binding protein 5
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP5 ELISA Kit images

  • Representative standard curve using ab100543


References for Human IGFBP5 ELISA Kit (ab100543)

ab100543 has not yet been referenced specifically in any publications.

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For both of these ELISA kits we do recommend that you make the wash buffer up fresh before every use and we haven't tested the long term stability of the buffer when diluted to 1X. It should be good if stored o...

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Thank you for your patient. I just had a response from my lab colleagues. They confirmed that all three kits were designed against the mature form of the protein (which is the full amino acid sequence minus the signalling peptide). As the epito...

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