Overview

  • Product nameHuman IGFBP5 full length protein
  • DescriptionHuman IGFBP5 full length protein

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMLGSFVHCEP CDEKALSMCP PSPLGCELVK EPGCGCCMTC ALAEGQSCGV YTERCAQGLR CLPRQDEEKP LHALLHGRGV CLNEKSYREQ VKIERDSREH EEPTTSEMAE ETYSPKIFRP KHTRISELKA EAVKKDRRKK LTQSKFVGGA ENTAHPRIIS APEMRQESEQ GPCRRHMEAS LQELKASPRM VPRAVYLPNC DRKGFYKRKQ CKPSRGRKRG ICWCVDKYGM KLPGMEYVDG DFQCHTFDSS NVS
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab49835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Inhibition Assay

    Functional Studies

    SDS-PAGE

  • Purity> 95 % by SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10mM Sodium citrate, pH 4
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

  • ReconstitutionReconstitute in water to a concentration of 0.1-1.0 mg/ml.

General info

  • Alternative names
    • IBP 5
    • IBP-5
    • IBP5
    • IBP5_HUMAN
    • IGF binding protein 5
    • IGF BP5
    • IGF-binding protein 5
    • IGFBP 5
    • IGFBP-5
    • IGFBP5
    • Insulin like growth factor binding protein 5
    • Insulin-like growth factor-binding protein 5
    see all
  • FunctionIGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Tissue specificityOsteosarcoma, and at lower levels in liver, kidney and brain.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P24593 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Human IGFBP5 full length protein images

  • Anti-IGFBP5 antibody (Biotin) (ab84229) at 0.1 µg/ml + Human IGFBP5 full length protein (ab49835) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Human IGFBP5 full length protein (ab49835)

This product has been referenced in:
  • Abrass CK & Hansen KM Insulin-like growth factor-binding protein-5-induced laminin gamma1 transcription requires filamin A. J Biol Chem 285:12925-34 (2010). Read more (PubMed: 20167606) »

See 1 Publication for this product

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