Overview

  • Product name
    Human IGFBP6 ELISA Kit
    See all IGFBP6 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 150 pg/ml
  • Range
    82.3 pg/ml - 60000 pg/ml
  • Recovery

    > 80 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 80.6 70% - 91%
    Serum 81.3 71% - 90%
    Plasma 83.5 72% - 92%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IGFBP6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP6 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IGFBP6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similarities
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications
    O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • IBP 6
    • IBP-6
    • IBP6
    • IBP6_HUMAN
    • IGF binding protein 6
    • IGF-binding protein 6
    • IGFBP 6
    • IGFBP-6
    • IGFBP6
    • Insulin like growth factor binding protein 6
    • Insulin-like growth factor-binding protein 6
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IGFBP6 ELISA Kit images

  • Representative standard curve using ab100544

  • Representative standard curve using ab100544

Protocols

References for Human IGFBP6 ELISA Kit (ab100544)

ab100544 has not yet been referenced specifically in any publications.

Product Wall

Thank you and your customer for your inquiry.

Although we have not tested the Human ab100540 and ab100544ELISA kits with ascite fluid,these kits are very accommodating to many different samples types so the ascites will most likely work.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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