Recombinant Anti-Human IgG antibody [EPR4421] (ab109489)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4421] to Human IgG
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Human IgG antibody [EPR4421]
See all Human IgG primary antibodies -
Description
Rabbit monoclonal [EPR4421] to Human IgG -
Host species
Rabbit -
Specificity
This antibody recognizes all four Human IgG isotypes (IgG1, IgG2, IgG3, and IgG4).
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human IgG2, IgG1, IgG3, and IgG4 recombinant proteins, human fetal thymus, fetal tonsil and fetal spleen tissue lysates. IHC-P: Human tonsil and stomach tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4421 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 52-58 kDa.
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IHC-P | (1) |
1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 52-58 kDa. |
IHC-P
1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- Omim: 147100 Human
- Omim: 147110 Human
- Omim: 147120 Human
- Omim: 147130 Human
see all -
Alternative names
- FLJ39988 antibody
- FLJ40036 antibody
- FLJ40253 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling Human IgG with purified ab109489 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/20000 dilution (purified)
Lane 1 : Human fetal thymus tissue lysate
Lane 2 : Human fetal tonsil tissue lysate
Lane 3 : Human fetal spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (unpurified)
Lane 1 : IgG2 recombinant protein (10 ng protein loaded)
Lane 2 : IgG1 recombinant protein (10 ng protein loaded)
Lane 3 : IgG3 recombinant protein (10 ng protein loaded)
Lane 4 : IgG4 recombinant protein (10 ng protein loaded) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (purified) + IgG3 recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/5000 dilution (purified)
Lane 1 : IgG1 recombinant protein
Lane 2 : IgG2 recombinant protein
Lane 3 : IgG4 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (301)
ab109489 has been referenced in 301 publications.
- Chen L et al. MALAT1-mediated EZH2 Recruitment to the GFER Promoter Region Curbs Normal Hepatocyte Proliferation in Acute Liver Injury. J Clin Transl Hepatol 11:97-109 (2023). PubMed: 36406327
- Wangzhou K et al. microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1. Oral Dis 29:491-504 (2023). PubMed: 34152066
- Zhang W et al. Oncogenic LINC00857 recruits TFAP2C to elevate FAT1 expression in gastric cancer. Cancer Sci 114:63-74 (2023). PubMed: 35524544
- Terzi A et al. A unique case of isolated thoracic spinal Rosai-Dorfman disease related to IgG4. Neuropathology 43:176-180 (2023). PubMed: 36226609
- Guo J et al. TIGAR deficiency induces caspase-1-dependent trophoblasts pyroptosis through NLRP3-ASC inflammasome. Front Immunol 14:1114620 (2023). PubMed: 37122710