Anti-Human IgG Fab fragment antibody [4A11] (ab771)
Key features and details
- Mouse monoclonal [4A11] to Human IgG Fab fragment
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Human IgG Fab fragment antibody [4A11] -
Description
Mouse monoclonal [4A11] to Human IgG Fab fragment -
Host species
Mouse -
Specificity
This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions. -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Human IgG Fab fragment.
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Positive control
- WB: Normal human immunoglobulins. Flow Cyt: Human peripheral blood cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4A11 -
Myeloma
unknown -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
Target
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Relevance
Human IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.
Images
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All lanes : Anti-Human IgG Fab fragment antibody [4A11] (ab771)
Lane 1 : Normal human immunoglobulins
Lane 2 : Normal bovine immunoglobulins
Performed under reducing conditions. -
Surface staining of human peripheral blood cells with ab771. An APC-conjugated goat anti-mouse IgG was used as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab771 has been referenced in 4 publications.
- Baart VM et al. Side-by-Side Comparison of uPAR-Targeting Optical Imaging Antibodies and Antibody Fragments for Fluorescence-Guided Surgery of Solid Tumors. Mol Imaging Biol 25:122-132 (2023). PubMed: 34642899
- Abd-Aziz N et al. A New Plant Expression System for Producing Pharmaceutical Proteins. Mol Biotechnol 62:240-251 (2020). PubMed: 32108286
- Lau YF et al. An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice. J Virol 84:8639-49 (2010). ELISA . PubMed: 20538850
- Drexler SK et al. SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of rheumatoid arthritis. Arthritis Rheum 62:2249-61 (2010). Flow Cyt ; Human . PubMed: 20506350