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|Sample type||Average %||Range|
|Serum||87||75% - 97%|
|Plasma||81.5||71% - 94%|
Abcam’s IgG1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG1 in serum and plasma.
This assay employs an antibody specific for Human IgG1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
|Components||1 x 96 tests|
|2,000X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||2 x 15ml|
|Biotinylated anti-Human IgG1||2 vials|
|IgG1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human IgG1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Human IgG1 measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum and plasma were diluted 1e6 - 64e6 fold. Human saliva and urine were diluted 1e3-64e3 fold.
Representative standard curve using ab100548
ab100548 has not yet been referenced specifically in any publications.