Overview

  • Product name
    Anti-Human IgG3 antibody [5G12]
    See all Human IgG3 primary antibodies
  • Description
    Mouse monoclonal [5G12] to Human IgG3
  • Specificity
    Subclass-specific for human IgG3. No allotype restriction. Hinge-region specific.
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    IgG3 from human serum.

  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Chromatography on protein A Sepharose
  • Clonality
    Monoclonal
  • Clone number
    5G12
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution. Specific antibody titer is 1/300 000 by indirect ELISA

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • DKFZp686H11213 antibody
      • FLJ39988 antibody
      • FLJ40036 antibody
      • FLJ40253 antibody
      • FLJ40587 antibody
      • FLJ40789 antibody
      • FLJ40834 antibody
      • G3m marker antibody
      • HDC antibody
      • Heavy chain disease protein antibody
      • Ig gamma 3 chain C region antibody
      • IgG3 antibody
      • IGHG 3 antibody
      • IGHG3 antibody
      • IGHG3 protein antibody
      • Immunoglobulin gamma 3 (Gm marker) antibody
      • Immunoglobulin gamma 3 antibody
      • Immunoglobulin heavy constant gamma 3 (G3m marker) antibody
      • Immunoglobulin heavy constant gamma 3 antibody
      • MGC45809 antibody
      see all

    References

    This product has been referenced in:
    • Poitelon Y  et al. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun 6:8303 (2015). WB ; Rat . Read more (PubMed: 26383514) »
    • Medeiros MM  et al. Natural antibody response to Plasmodium falciparum merozoite antigens MSP5, MSP9 and EBA175 is associated to clinical protection in the Brazilian Amazon. BMC Infect Dis 13:608 (2013). ELISA ; Human . Read more (PubMed: 24373342) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. I have listed the concentrations below. Cat.# ab1928, lot 81191, conc. 3.00 mg/ml Cat.# ab1935, lot 74364, conc. 5.00 mg/ml Cat.# ab1930, lot 81196, conc. 6.30 mg/ml Cat.# ab1927, lot81198, conc. 3.70 mg/ml

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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