Overview

  • Product nameAnti-Human IgG3 antibody
    See all Human IgG3 primary antibodies
  • Description
    Mouse polyclonal to Human IgG3
  • SpecificityThis antibody reacts with the IgG3 heavy chain constant region.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human Human IgG3 aa 1-100. Vector coding for a partial recombinant fusion protein, corresponding to amino acids 1-100 of Human IgG3.
    Sequence:

    ASTKGPSVFP LAPCSRSTSG GTAALGCLVK DYFPEPVTVS WNSGALTSGV HTFPAVLQSS GLYSLSSVVT VPSSSLGTQT YTCNVNHKPS NTKVDKRVEL


    Database link: P01860

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab43683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000.
    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceIgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • DKFZp686H11213 antibody
      • FLJ39988 antibody
      • FLJ40036 antibody
      • FLJ40253 antibody
      • FLJ40587 antibody
      • FLJ40789 antibody
      • FLJ40834 antibody
      • G3m marker antibody
      • HDC antibody
      • Heavy chain disease protein antibody
      • Ig gamma 3 chain C region antibody
      • Ig gamma 3 chain C region antibody
      • IgG3 antibody
      • IGHG 3 antibody
      • IGHG3 antibody
      • IGHG3 protein antibody
      • Immunoglobulin gamma 3 (Gm marker) antibody
      • Immunoglobulin gamma 3 antibody
      • Immunoglobulin heavy constant gamma 3 (G3m marker) antibody
      • Immunoglobulin heavy constant gamma 3 antibody
      • MGC45809 antibody
      see all

    Anti-Human IgG3 antibody images

    • All lanes : Anti-Human IgG3 antibody (ab43683) at 1/1000 dilution

      Lane 1 : 20µg of a total protein extract from E coli with ~50ng to 500ng of a fusion protein of an irrelevant antigen (negative control lane).
      Lane 2 : 20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (fusion protein).

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 57 kDa

    References for Anti-Human IgG3 antibody (ab43683)

    ab43683 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"