Recombinant Anti-Human IgG3 antibody [EPR4419] (ab109761)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4419] to Human IgG3
- Suitable for: WB, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-Human IgG3 antibody [EPR4419]
See all Human IgG3 primary antibodies -
Description
Rabbit monoclonal [EPR4419] to Human IgG3 -
Host species
Rabbit -
Specificity
ab109761 does not cross-react with human IgG1, IgG2, and IgG4. -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IgG3 recombinant protein, Human tonsil and Human plasma lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4419 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000.
|
|
IP |
1/10 - 1/100.
|
Notes |
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WB
1/1000 - 1/10000. |
IP
1/10 - 1/100. |
Target
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Relevance
IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). -
Cellular localization
Secreted -
Database links
- Entrez Gene: 3502 Human
- Omim: 147120 Human
- SwissProt: P01860 Human
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Alternative names
- DKFZp686H11213 antibody
- FLJ39988 antibody
- FLJ40036 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab109761 has been referenced in 11 publications.
- Melani RD et al. Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire. J Proteome Res 21:274-288 (2022). PubMed: 34878788
- Zhang F et al. Hsa_circ_0010235 functions as an oncogenic drive in non-small cell lung cancer by modulating miR-433-3p/TIPRL axis. Cancer Cell Int 21:73 (2021). PubMed: 33494763
- Liu J et al. Circ_0105346 Knockdown Inhibits Osteosarcoma Development via Regulating miR-1182/WNT7B Axis. Cancer Manag Res 13:521-535 (2021). PubMed: 33505171
- Liu Y et al. MicroRNA-124 facilitates lens epithelial cell apoptosis by inhibiting SPRY2 and MMP-2. Mol Med Rep 23:N/A (2021). PubMed: 33760112
- Meng X et al. Silencing of the Long Non-Coding RNA TTN-AS1 Attenuates the Malignant Progression of Osteosarcoma Cells by Regulating the miR-16-1-3p/TFAP4 Axis. Front Oncol 11:652835 (2021). PubMed: 34141611