Recombinant

Anti-Human IgM antibody [RM121] (ab193159)

Overview

  • Product name
    Anti-Human IgM antibody [RM121]
    See all Human IgM primary antibodies
  • Description
    Rabbit monoclonal [RM121] to Human IgM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Sandwich ELISA, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Human IgM. Human IgM
    Database link: P01871

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Protein A affinity purified from an animal origin–free culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    RM121
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Use: 50ng/well – 200ng/well (for Capture);
0.05μg/mL – 0.2μg/mL (for Detection);

ICC/IF Use a concentration of 0.5 - 2 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 0.5 - 2 µg/ml.

Target

  • Relevance
    IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
  • Cellular localization
    Cell Membrane and Secreted
  • Database links
  • Alternative names
    • Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region antibody
    • Immunoglobin heavy chain constant region mu antibody
    • AGM1 antibody
    • Constant region of heavy chain of IgM antibody
    • DKFZp686I15196 antibody
    • DKFZp686I15212 antibody
    • FLJ00385 antibody
    • Ig mu chain C region antibody
    • IGHM antibody
    • IgM heavy chain constant region antibody
    • Immunoglobin heavy constant mu antibody
    • Immunoglobulin mu antibody
    • MGC104996 antibody
    • MGC52291 antibody
    • MU antibody
    • VH antibody
    see all

Images

  • A titer ELISA using ab193159. The plate was coated with different amounts of human IgM. A serial dilution of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

  • ELISA of human immunoglobulins shows ab193159 only reacted to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

References

ab193159 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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