Anti-Human IgM Fc fragment antibody [CH2] (ab772)

Overview

  • Product nameAnti-Human IgM Fc fragment antibody [CH2]
    See all Human IgM Fc fragment primary antibodies
  • Description
    Mouse monoclonal [CH2] to Human IgM Fc fragment
  • SpecificityThis antibody reacts with Fc fragment of human IgM. Specificity was confirmed by Western blotting analysis of purified human IgM, under reducing conditions, and also by immunofluorescence staining of Daudi cells
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by DEAE chromatography and precipitation methods.
  • ClonalityMonoclonal
  • Clone numberCH2
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Flow Cyt
WB
ELISA
  • Application notesELISA: Use at an assay dependant concentration.
    Flow Cyt: Use at an assay dependant concentration.
    IF: Use at an assay dependant concentration.
    WB: Use at an assay dependant concentration.
    This antibody blocks binding of standard antibody to Fc fragment of human IgM and does not block binding of standard antibody to Fab fragment of human IgM.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceImmunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
    • Cellular localizationCell Membrane and Secreted
    • Database links
    • Alternative names
      • DKFZp686I15196 antibody
      • DKFZp686I15212 antibody
      • FLJ00385 antibody
      • MGC104996 antibody
      • MGC52291 antibody
      • MU antibody
      • mu heavy chain constant region antibody
      • VH antibody
      see all

    References for Anti-Human IgM Fc fragment antibody [CH2] (ab772)

    ab772 has not yet been referenced specifically in any publications.

    Product Wall

    The originator of these two antibodies has informed me that the anti Fab fragment antibody is better (IgG receptors bind IgG via Fc so it may be occupied). The Fc fragment is not good for IgM bound to the B-cell surface, but is great for soluble IgM.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"