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|Sample type||Average %||Range|
|Serum||102.2||15.6pg/ml - 500pg/ml|
Abcam’s Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.
A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.
This kit will recognize both endogenous and recombinant Human IL-1 beta.
|Components||Identifier||2 x 96 tests||1 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||2 x 10ml||1 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 13ml||1 x 7.5ml|
|Biotinylated anti-IL-1 beta||Red||2 x 400µl||1 x 400µl|
|Chromogen TMB Substrate Solution||1 x 24ml||1 x 11ml|
|Control||Silver||4 vials||2 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|IL-1 beta Microplate (12 x 8 well strips)||2 units||1 unit|
|IL-1 beta Standard (Lyophilized)||Yellow||4 vials||2 vials|
|Standard Diluent (Serum)||2 x 7ml||1 x 7ml|
|Stop Reagent||Black||2 x 11ml||1 x 11ml|
|Streptavidin-HRP||4 x 5µl||2 x 5µl|
Our Abpromise guarantee covers the use of ab46052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).
IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and 1 ug x mL-1 LPS (Sigma) for the last 6 hours (P+L).
Representative Standard Curve using ab46052