Human IL-1 beta ELISA Kit (Interleukin-1 beta) (ab46052)

Overview

  • Product nameHuman IL-1 beta ELISA Kit (Interleukin-1 beta)
    See all IL1 beta kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    6 4.5%
    Inter-assay
    Sample n Mean SD CV%
    6 8.7%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    6.5 pg/ml
  • Range
    15.6 pg/ml - 500 pg/ml
  • Recovery

    102.2 %

    Sample specific recovery
    Sample type Average % Range
    Serum 102.2 15.6pg/ml - 500pg/ml

  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human IL-1 beta ab46052 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 beta in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for IL-1 beta has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-1 beta concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-1 beta are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-1 beta present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-1 beta.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similaritiesBelongs to the IL-1 family.
  • DomainThe similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localizationSecreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-1 beta ELISA Kit (Interleukin-1 beta) images

  • Standard curve of human IL-1β in standard diluent with background signal subtracted (duplicates; +/- SD).

  • IL-1β detected in supernatants from control THP-1 cells (-) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) and 1 ug x mL-1 LPS (Sigma) for the last 6 hours (P+L).

  • Representative Standard Curve using ab46052

Protocols

References for Human IL-1 beta ELISA Kit (Interleukin-1 beta) (ab46052)

ab46052 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

If you diluted the controls in the recommended volume of diluent, then you should have ended up with the concentration that is indicated on the control vials. Was there no concentration listed on the vials? If there ...

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Thank you for contacting Abcam.

For all three of those ELISA kits, there are standards and controls (4 vials of control per kit)provided and you should reconstitute those samples in the correct diluent depending on your sample types.
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Gracias por contactarnos.


He querido comprobar la posibilidad de presentar reactividad cruzada entre ratón y humano en los kits contra las citoquinas que mencionas.

Tenemos varios kits contra dichas citoquinas espec&iacu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"