Overview

  • Product nameHuman IL-1 R4 ELISA Kit (ST2)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    1.65 pg/ml - 1200 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.43 91% - 105%
    Serum 109.6 98% - 117%
    Plasma 84.06 67% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-1 R4 (ST2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 R4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-1 R4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 R4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 R4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceST2 is a member of a superfamily containing the Interleukin 1 (IL1) receptor and the Toll-like receptors (TLRs). The TLRs are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. ST2 was originally identified as a protein whose production was stimulated by various proliferation-inducing agents such as PDGF and FGF. More recently, it has been shown to negatively regulate IL1 receptor and Toll-like receptor 4 (TLR4) signaling and to maintain endotoxin tolerance. It has been suggested that the inhibition of TLR4 signaling occurs through the association and sequestering of TLR adaptor molecules such as MyD88 and TIRAP.
  • Alternative names
    • DER4
    • FIT 1
    • Growth stimulation expressed
    • Homolog of mouse growth stimulation expressed
    • IL-1 R4
    • IL1R4
    • IL33R
    • Interleukin 1 receptor like 1
    • Interleukin 1 receptor related protein
    • Protein ST2
    • ST2S
    • T1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-1 R4 ELISA Kit (ST2) images

  • Representative standard curve using ab100563

  • Representative standard curve using ab100563

Protocols

References for Human IL-1 R4 ELISA Kit (ST2) (ab100563)

ab100563 has not yet been referenced specifically in any publications.

Product Wall

The detection range for ab100563 Human IL-1 R4 ELISA Kit (ST2) is based on the standard curve range and from testing of normal human serum with the kit.  Levels of IL-1 R4 in these samples using this kit were about 150 pg/ml).

Each kit manufac...

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The kit ab100563 recognizes the extracellular domain on the protein, aa 19-328.



J’ai contacté le laboratoire concernant le kit ab100563. L'immunogène utilisé pour créer les anticorps est une protéine recombinant humain IL-1R4 qui correspond à la région extracellulair...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"