• Product nameHuman IL-10 ELISA Kit (Interleukin-10)
    See all IL10 kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 12%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    2.34 pg/ml - 150 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.37 87% - 110%
    Serum 101.94 87% - 108%
    Plasma 102.39 93% - 109%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-10 ELISA Kit (Interleukin-10) images

  • IL-10 measured in culture supernatants from control (-) or activated cell lines (dilution range 1/1-1/00), stimulated for 24 hours with 50 ng x mL-1 of PMA (P)(ab120297) and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours or in PBMC supernatants (dilution range 1/10-1/1000), from control or treated cells ((PHA) 48 hours with 2% PHA-M; LifeTechnologies) (duplicates +/- SD).

  • Standard curve (assay diluent B) with background signal subtracted (duplicates; +/- SD).

  • Standard curve (assay diluent A) with background signal subtracted (duplicates; +/- SD).

  • Dilution curves (in assay diluent B) of human IL-10 (ab100549) and NIBSC standard (93/722). One ng of standard IL-10 corresponds to 0.94 (+/- 0.08) U NIBSC standard. Background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100549

  • Representative standard curve using ab100549


References for Human IL-10 ELISA Kit (Interleukin-10) (ab100549)

This product has been referenced in:
  • Andersen CJ  et al. Egg intake during carbohydrate restriction alters peripheral blood mononuclear cell inflammation and cholesterol homeostasis in metabolic syndrome. Nutrients 6:2650-67 (2014). Human . Read more (PubMed: 25045936) »

See 1 Publication for this product

Product Wall

I have broken down the cross-reactivity information for each kit below:

· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected ...

Read More