Human IL-10 ELISA Kit (Interleukin-10) (ab108871)

Overview

  • Product nameHuman IL-10 ELISA Kit (Interleukin-10)
    See all IL10 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.4%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay typeSandwich (quantitative)
  • Sensitivity
    > 0.12 ng/ml
  • Range
    0.125 ng/ml - 8 ng/ml
  • Recovery

    98 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-10 (Interleukin-10) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 levels in cell culture supernatants, serum, plasma and tissue extracts.

    An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate. This assay recognizes both natural and recombinant Human IL-10.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-10 ELISA Kit (Interleukin-10) images

  • Representative Standard Curve Using ab108871.

Protocols

References for Human IL-10 ELISA Kit (Interleukin-10) (ab108871)

ab108871 has not yet been referenced specifically in any publications.

Product Wall

The equivalent IL10 assay, with 12 strips of 8 wells each, is ab100549. Product ab108871 comes with a single plate, not strips, but does use an HRP-conjugated streptavidin, as does the TNF alpha ELISA, ab100654.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"