Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity (ab46059)

Overview

  • Product nameHuman IL-10 ELISA Kit (Interleukin-10) High Sensitivity
    See all IL10 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3 7.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 10.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1.3 pg/ml
  • Range
    1.56 pg/ml - 50 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94 % - %
    Serum 80 59% - 93%

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-10 (Interleukin-10) Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in Human sera, plasmas, buffered solutions and cell culture media.


    A monoclonal antibody specific for IL-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-10 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-10.


    Save time with a 90-minute ELISA protocol. Try Human IL-10 ELISA Kit (Interleukin-10) (ab185986) from our SimpleStep ELISA® range.


     


     

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Tissue specificityProduced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • Sequence similaritiesBelongs to the IL-10 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity images

  • Representative Standard Curve using ab46059

Protocols

References for Human IL-10 ELISA Kit (Interleukin-10) High Sensitivity (ab46059)

This product has been referenced in:
  • Hasegawa T  et al. Chafuroside B, an Oolong Tea Polyphenol, Ameliorates UVB-Induced DNA Damage and Generation of Photo-immunosuppression Related Mediators in Human Keratinocytes. PLoS One 8:e77308 (2013). Sandwich ELISA ; Human . Read more (PubMed: 24116222) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.

I am sorry about the continued problems that you have been experiencing using this kit. Unfortunately I do not have any further suggestions to help with this issue. I have created a credit note/refund for the o...

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I would like to suggest that you try using another kit to determine whether this is a product specific issue. If you provide me with the orginal Abcam order confirmation code I can send you a free of charge replacement for this product.

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I've passed this information on to the lab for you.

They feel that the data enclosed are pretty good, except the blank which is indeed a little bit high, and which should rather be considered as a zero. They also also would like to confirm w...

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Thank you for that additional information. The matrix effect could be called quenching. The high protein concentration of serum samples or components contained in samples can affect assay response to the analyte (IL-10) and give background. If yo...

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Thank you for contacting Abcam.

I have had the opportunity to speak with the lab regarding this issue. The low signal obtained may be linked to the use of human serum as standard curve diluent. Have you diluted the standards in human serum o...

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Thank you for your call today, and for letting us know about the trouble with this kit. As we discussed, please forward any data you have using this kit, and I will see if there are any suggestions that we can make to improve the results. I look forwar...

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Thank you for your call yesterday and for your patience while i have been in touch with the lab regarding these IL10 kits. Here is the reply that I received from my contact at the lab: "To be on the safe side regarding sensitivity, my opinion would be ...

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