Human IL-10 RB ELISA Kit (Interleukin-10 Receptor beta) (ab100550)

Overview

  • Product nameHuman IL-10 RB ELISA Kit (Interleukin-10 Receptor beta)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 129.1 120% - 140%
    Serum 121.9 110% - 129%
    Plasma 104.5 92% - 112%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-10 RB (Interleukin 10 Receptor beta) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human IL-10 RB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-10 RB coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 RB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 RB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 RB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionShared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29.
  • Involvement in diseaseDefects in IL10RB are the cause of inflammatory bowel disease type 25 (IBD25) [MIM:612567]. It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • Sequence similaritiesBelongs to the type II cytokine receptor family.
    Contains 2 fibronectin type-III domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • CDw210b
    • CRF2 4
    • CRF2-4
    • CRFB4
    • Cytokine receptor class II member 4
    • Cytokine receptor class-II member 4
    • Cytokine receptor family 2 member 4
    • D21S58
    • D21S66
    • I10R2_HUMAN
    • IL-10 receptor subunit beta
    • IL-10R subunit 2
    • IL-10R subunit beta
    • IL-10R2
    • IL-10RB
    • IL10 receptor subunit beta
    • IL10R subunit 2
    • IL10R subunit beta
    • IL10R2
    • IL10RB
    • Interleukin 10 receptor subunit 2
    • Interleukin 10 receptor subunit beta
    • Interleukin-10 receptor subunit 2
    • Interleukin-10 receptor subunit beta
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-10 RB ELISA Kit (Interleukin-10 Receptor beta) images

  • Representative standard curve using ab100550

  • Representative standard curve using ab100550

Protocols

References for Human IL-10 RB ELISA Kit (Interleukin-10 Receptor beta) (ab100550)

ab100550 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab100550.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"