Human IL-12 ELISA Kit (Interleukin-12) High Sensitivity (ab46035)


  • Product nameHuman IL-12 ELISA Kit (Interleukin-12) High Sensitivity
    See all IL12 kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Sample A 8 16.1pg/ml 1.25 7.8%
    Sample B 8 6.1pg/ml 0.4 6.6%
    Sample n Mean SD CV%
    Sample A 20 16pg/ml 1.2 7.9%
    Sample B 20 6pg/ml 0.6 9.5%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.75 pg/ml
  • Range
    0.78 pg/ml - 25 pg/ml
  • Recovery

    89 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-12 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-12.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CLMF p35
    • CLMF p40
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL 12A
    • IL 12B
    • IL-12 subunit p35
    • IL-12A
    • IL12 p35
    • IL12 p40
    • IL12A
    • IL12A_HUMAN
    • Interleukin 12 alpha chain
    • Interleukin 12 beta chain
    • Interleukin-12 subunit alpha
    • NK cell stimulatory factor chain 1
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF1
    • NKSF2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab46035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-12 ELISA Kit (Interleukin-12) High Sensitivity images

  • Representative Standard Curve using ab46035


References for Human IL-12 ELISA Kit (Interleukin-12) High Sensitivity (ab46035)

This product has been referenced in:
  • Pham PV  et al. Targeting breast cancer stem cells by dendritic cell vaccination in humanized mice with breast tumor: preliminary results. Onco Targets Ther 9:4441-51 (2016). Read more (PubMed: 27499638) »
  • Pham PV  et al. A simple in vitro method for evaluating dendritic cell-based vaccinations. Onco Targets Ther 7:1455-64 (2014). Sandwich ELISA . Read more (PubMed: 25170272) »

See all 2 Publications for this product

Product Wall


The kit is suitable for tissue homogenates.
2) The suggested homogenate buffer containing 0.5% Tween20, EDTA and Tris is appropriate and compatible with the kit.