Human IL-12 + IL-23 p40 ELISA Kit (ab119534)

Overview

  • Product name
    Human IL-12 + IL-23 p40 ELISA Kit
    See all IL12 + IL23 p40 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 6.4%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    71 %

    Sample specific recovery
    Sample type Average % Range
    Serum 71 65% - 75%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 + IL-23 p40 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-12 + IL-23 p40 concentrations in Cell culture supernatant, serum and plasma (citrate, heparin).

    Human IL-12 + IL-23 p40 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-12 + IL-23 p40 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-12 + IL-23 p40 captured on the plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-Human IL12 + IL23 p40 monoclonal antibody 1 x 70µl
    Human IL-12 + IL-23 p40 Standard Lyophilized (4 ng/mL on reconstitution) 2 vials
    Microplate coated with anti-Human IL-12 + IL-23 p40 polyclonal antibodies (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Relevance
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Alternative names
    • CLMF
    • CLMF p40
    • CLMF2
    • Cytotoxic lymphocyte maturation factor 2
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL 12 subunit p40
    • IL 12B
    • IL12 subunit p40
    • IL12B
    • Interleukin 12 beta chain
    • Interleukin 12 p40
    • Interleukin 12 subunit beta
    • Interleukin 12B
    • Natural killer cell stimulatory factor 2
    • Natural killer cell stimulatory factor 40 kD subunit
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF2
    • p40
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119534.

Protocols

References

ab119534 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your inquiry.

Unfortunately, the supplier does not epitope map their antibodies; therefore, they are unable to provide the specific domain that the anti-p40 antibody ab119534 binds to.

We aim to provide as much informati...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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