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|Sample type||Average %||Range|
|Cell culture supernatant||127.6||120% - 134%|
|Serum||99.73||91% - 108%|
|Plasma||91.11||85% - 98%|
Abcam’s IL-12 P40 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 P40 in serum (Human IL-12 p40 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-12 P40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 P40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 P40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 P40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||Identifier||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Human IL-12 P40||Item F||2 vials|
|IL-12 P40 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human IL-12 P40 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113324 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"