Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab100552)

Overview

  • Product name
    Human IL-12 p70 ELISA Kit (Interleukin-12 p70)
    See all IL12 p70 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.8 pg/ml - 600 pg/ml
  • Recovery

    88 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.52 88% - 109%
    Plasma 85.63 79% - 101%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 p70 (Interleukin-12 p70) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in plasma and cell culture supernatants.


     This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-12 p70 in normal serum with ab100552, only in serum spiked with Human IL-12 p70.


    Get results in 90 minutes with Human IL-12p40/p70 ELISA Kit (ab178009) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-12 p70 (lyophilized) 2 vials
    IL-12 p70 Microplate (12 x 8 wells) 1 unit
    Recombinant IL-12 p70 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100552

  • Representative standard curve using ab100552

Protocols

References

ab100552 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. Low signal in this case may be the result of incubation time. I would recommend that you increase the incubation period of assay step #2 to overnight. I also recommend that you prepare all reagents as you use them, especial...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up