Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab100552)

Overview

  • Product nameHuman IL-12 p70 ELISA Kit (Interleukin-12 p70)
    See all IL12 p70 kits
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.8 pg/ml - 600 pg/ml
  • Recovery

    88 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.52 88% - 109%
    Plasma 85.63 79% - 101%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 p70 (Interleukin-12 p70) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p70 in plasma and cell culture supernatants.


     This assay employs an antibody specific for Human IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-12 p70 in normal serum with ab100552, only in serum spiked with Human IL-12 p70.


    Get results in 90 minutes with Human IL-12p40/p70 ELISA Kit (ab178009) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-12 p70 ELISA Kit (Interleukin-12 p70) images

  • Representative standard curve using ab100552

  • Representative standard curve using ab100552

Protocols

References for Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab100552)

ab100552 has not yet been referenced specifically in any publications.

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Thank you for contacting us. Low signal in this case may be the result of incubation time. I would recommend that you increase the incubation period of assay step #2 to overnight. I also recommend that you prepare all reagents as you use them, especial...

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