Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab119532)

Overview

  • Product name
    Human IL-12 p70 ELISA Kit (Interleukin-12 p70)
    See all IL12 p70 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 4.8%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    2.1 pg/ml
  • Range
    3.1 pg/ml - 200 pg/ml
  • Recovery

    103 %

    Sample specific recovery
    Sample type Average % Range
    Serum 103 79% - 118%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 p70 (Interleukin-12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human IL-12 p70 concentrations in Cell culture supernatant serum, and plasma (EDTA, citrate, heparin).


    Human IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human IL-12 p70 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human IL-12 p70 captured on the plate.


    Get results in 90 minutes with Human IL-12p40/p70 ELISA Kit (ab178009) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human IL12 p70 monoclonal antibody 1 x 100µl
    Control high 1 vial
    Control low 1 vial
    IL-12 p70 Standard, lyophilized (400 pg/mL on reconstitution) 2 vials
    Microplate coated with antibody to Human IL-12 p70 (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Function
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119532.

Protocols

References

ab119532 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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