Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab46143)

Overview

  • Product nameHuman IL-12 p70 ELISA Kit (Interleukin-12 p70)
    See all IL12 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6 8.9%
  • Tests
    2 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 2.2 pg/ml
  • Range
    6.25 pg/ml - 200 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Serum 91 86% - 94%

  • Assay time
    3h 40m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-12 p70 (Interleukin- 12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 in supernatants, buffered solutions or serum and plasma samples.

    A monoclonal antibody specific IL-12 p70 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 p70 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 p70 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 p70 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-12 p70.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CLMF p35
    • CLMF p40
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL 12A
    • IL 12B
    • IL-12 subunit p35
    • IL-12A
    • IL12 p35
    • IL12 p40
    • IL12A
    • IL12A_HUMAN
    • Interleukin 12 alpha chain
    • Interleukin 12 beta chain
    • Interleukin-12 subunit alpha
    • NK cell stimulatory factor chain 1
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF1
    • NKSF2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-12 p70 ELISA Kit (Interleukin-12 p70) images

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    Representative Standard Curve using ab46143

Protocols

References for Human IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab46143)

ab46143 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"