Human IL-13 ELISA Kit (Interleukin-13) (ab100553)

Overview

  • Product nameHuman IL-13 ELISA Kit (Interleukin-13)
    See all IL13 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.15 pg/ml
  • Range
    0.16 pg/ml - 40 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100.65 88% - 107%
    Serum 96.35 84% - 103%
    Plasma 94.39 82% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-13 (Interleukin-13) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-13 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human IL-13 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in diseaseAllergic rhinitis
  • Sequence similaritiesBelongs to the IL-4/IL-13 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC30
    • P600
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-13 ELISA Kit (Interleukin-13) images

  • Representative standard curve using ab100553

  • Representative standard curve using ab100553

Protocols

References for Human IL-13 ELISA Kit (Interleukin-13) (ab100553)

ab100553 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your calls this week and I appreciate your patience. Unfortunately, all of the components of our kits are not available for individual purchase, which includes the detection antibody of ab100533. We can only provide this component...

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Thanks for your email. Sorry for the delay. I am looking into the possibility of providing this antibody to you separately from ab100553. Currently it is not sold separately and is only sold as part of the IL13 Human ELISA Kit you purchased. I w...

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Thank you for your email.  I have addressed your questions below.   1) The protocol mentions that the biotinilated IL13 detection antibody can be stored for only 5 days at 4 degrees C once resuspended with 100ul buffer. This sounds like a short tim...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"