Human IL-13 ELISA Kit (Interleukin-13) (ab46038)

Overview

  • Product nameHuman IL-13 ELISA Kit (Interleukin-13)
    See all IL13 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 99.04pg/ml 0.476 0.48%
    Sample B 8 13.47pg/ml 0.423 3.14%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 58 99.95pg/ml 1.439 1.43%
    Sample B 58 13.27pg/ml 0.767 5.77%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1.5 pg/ml
  • Range
    3.12 pg/ml - 100 pg/ml
  • Recovery

    102.3 %

  • Assay time
    2h 10m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-13 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-13 in Human serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for IL-13 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-13.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • Involvement in diseaseAllergic rhinitis
  • Sequence similaritiesBelongs to the IL-4/IL-13 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness 1 (bronchial asthma)
    • IL 13
    • IL-13
    • Il13
    • IL13_HUMAN
    • Interleukin 13
    • Interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • NC30
    • P600
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human IL-13 ELISA Kit (Interleukin-13) images

  • Representative Standard Curve using ab46038

Protocols

References for Human IL-13 ELISA Kit (Interleukin-13) (ab46038)

ab46038 has not yet been referenced specifically in any publications.

Product Wall

I received confirmation from the laboratory that samples/standards are co-incubate with the biotinylated Antibody (1h30 room temperature) in the IL-13 ELISA Kit. There's then a washing step and the incubation of the streptavidin-HRP as detailed in t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"