Overview

  • Product name
    Human IL-13 Receptor alpha ELISA Kit
    See all IL13 receptor alpha 2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 14 3024pg/ml 93.67 3.1%
    B 14 1054pg/ml 29.69 2.82%
    Inter-assay
    Sample n Mean SD CV%
    A 8 2744pg/ml 90.1 3.28%
    B 8 725pg/ml 43.9 6.06%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 150 pg/ml
  • Range
    156 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 156pg/ml - 5000pg/ml

  • Assay time
    3h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-13 Receptor alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interleukin 13 Receptor (IL-13 Receptor alpha, also known as CD213 alpha 2) in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-13 Receptor alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-13 Receptor alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-13 Receptor alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-13 Receptor alpha present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-13 Receptor alpha.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-13 Receptor alpha Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-13 Receptor alpha Microplate (12 x 8 well strips) 1 unit 2 units
    IL-13 Receptor alpha Standard (Lyophilized) Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Function
    Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 5 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • Cancer/testis antigen 19
    • CD213a2
    • CT19
    • I13R2_HUMAN
    • IL-13 receptor subunit alpha-2
    • IL-13R subunit alpha-2
    • IL-13R-alpha-2
    • IL-13RA2
    • IL13 receptor
    • IL13BP
    • IL13R
    • Il13ra2
    • Interleukin 13 binding protein
    • Interleukin 13 receptor alpha 2 chain
    • Interleukin-13 receptor subunit alpha-2
    • Interleukin-13-binding protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab46066

Protocols

References

ab46066 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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