Overview

  • Product name
    Human IL-16 ELISA Kit (Interleukin-16)
    See all IL16 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    104 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 107.5 97% - 117%
    Serum 113.7 103% - 122%
    Plasma 92.26 80% - 112%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-16 (Interleukin-16) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-16 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-16 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human IL-16 ELISA Kit (ab193763) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 2 x 15ml
    Biotinylated anti-Human IL-16 2 vials
    IL-16 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-16 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
    Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
    Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
  • Tissue specificity
    Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.
  • Sequence similarities
    Contains 4 PDZ (DHR) domains.
  • Post-translational
    modifications
    Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
  • Cellular localization
    Cytoplasm; Cytoplasm. Nucleus and Secreted.
  • Information by UniProt
  • Alternative names
    • FLJ16806
    • FLJ42735
    • FLJ44234
    • HGNC:5980
    • HsT19289
    • IL-16
    • IL16
    • IL16_HUMAN
    • Interleukin 16
    • Interleukin 16 precursor
    • Interleukin-16
    • KIAA4048
    • LCF
    • Lymphocyte chemoattractant factor
    • mKIAA4048
    • Neuronal interleukin 16
    • NIL16
    • OTTHUMP00000185571
    • OTTHUMP00000190849
    • OTTHUMP00000190850
    • PrIL16
    • Pro interleukin 16
    • Prointerleukin 16
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100555

Protocols

References

ab100555 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab100555.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up